"Research efforts in the laboratory are directed towards developing a deeper
understanding of various cellular RNA transactions, primarily using two model
systems - bakers yeast Saccharomyces cerevisiae and higher plant Arabidopsis
thaliana. We also continue to explore all scientific directions previously headed by Dr.
Dmitry Belostotsky (Current Opinion in Cell Biology Obituary, ASPB News Obituary)."
"Multiple quality control steps ensure that only fully processed and correctly
assembled mRNP complexes are released from the site of transcription and exported
into the cytoplasm. On the other hand, aberrant mRNPs are retained in the vicinity of
the sites of transcription and ultimately degraded. Despite the widespread occurrence
of such proofreading mechanisms from yeast to humans, mechanisms behind these
quality control checkpoints remain poorly understood. On the yeast side of the lab,
we seek to combine the yeast genetics and genomics with deconvolution
microscopy-based imaging to obtain better insight into these processes."
"On the plant side, our efforts are directed towards understanding of the role of
exosome complex in regulation of various steps of gene expression. The exosome
complex is a conserved, functionally versatile macromolecular machine known to play
a central and essential role in RNA metabolism both in the nucleus and in the
cytoplasm. Using whole-transcriptome tiling microarrays we obtained first
genome-wide exosome transcript maps which led to identification of a tightly
repressed transcriptome that can only be visualized through transient inhibition of
exosome activity. Our laboratory is pursuing further in depth examination of the role
exosome complex plays in regulation of expression of this "hidden" layer of the
transcriptome."
Our work is supported by NIH, USDA, NSF, BARD and Missouri Life Science Research
Board.
RECENT PAPERS FROM THE LAB:
Methods Mol Biol. 553: 39-56 (2009)
RNA Biol. 6: 446-54 (2009)
Curr Opin Cell Biol. 21: 352-8 (2009)
Curr Opin Plant Biol. 12 : 96-10 (2009)
Curr Top Microbiol Immunol. : 326:179-99 (2008)
Methods Enzymol. 448: 429-43 (2008)
RNA 14: 66-77 (2008)
Cell, 131: 1340-1353 (2007) - Selected as "Must Read" by the Faculty of 1000
(www.f1000biology.com/article/id/1100142/evaluation).
EMBO J 25: 4253-4262 (2006)
Trends Plant Sci 10: 347-353 (2005)
Mol Cell 16: 498-500 (2004)
RNA 10: 1200-1214 (2004) - Selected by the Faculty of 1000
(www.facultyof1000.com/article/15247430/evaluation)
RNA 9: 1476-1490 (2003)
Genetics 163: 311-319 (2003)
understanding of various cellular RNA transactions, primarily using two model
systems - bakers yeast Saccharomyces cerevisiae and higher plant Arabidopsis
thaliana. We also continue to explore all scientific directions previously headed by Dr.
Dmitry Belostotsky (Current Opinion in Cell Biology Obituary, ASPB News Obituary)."
"Multiple quality control steps ensure that only fully processed and correctly
assembled mRNP complexes are released from the site of transcription and exported
into the cytoplasm. On the other hand, aberrant mRNPs are retained in the vicinity of
the sites of transcription and ultimately degraded. Despite the widespread occurrence
of such proofreading mechanisms from yeast to humans, mechanisms behind these
quality control checkpoints remain poorly understood. On the yeast side of the lab,
we seek to combine the yeast genetics and genomics with deconvolution
microscopy-based imaging to obtain better insight into these processes."
"On the plant side, our efforts are directed towards understanding of the role of
exosome complex in regulation of various steps of gene expression. The exosome
complex is a conserved, functionally versatile macromolecular machine known to play
a central and essential role in RNA metabolism both in the nucleus and in the
cytoplasm. Using whole-transcriptome tiling microarrays we obtained first
genome-wide exosome transcript maps which led to identification of a tightly
repressed transcriptome that can only be visualized through transient inhibition of
exosome activity. Our laboratory is pursuing further in depth examination of the role
exosome complex plays in regulation of expression of this "hidden" layer of the
transcriptome."
Our work is supported by NIH, USDA, NSF, BARD and Missouri Life Science Research
Board.
RECENT PAPERS FROM THE LAB:
Methods Mol Biol. 553: 39-56 (2009)
RNA Biol. 6: 446-54 (2009)
Curr Opin Cell Biol. 21: 352-8 (2009)
Curr Opin Plant Biol. 12 : 96-10 (2009)
Curr Top Microbiol Immunol. : 326:179-99 (2008)
Methods Enzymol. 448: 429-43 (2008)
RNA 14: 66-77 (2008)
Cell, 131: 1340-1353 (2007) - Selected as "Must Read" by the Faculty of 1000
(www.f1000biology.com/article/id/1100142/evaluation).
EMBO J 25: 4253-4262 (2006)
Trends Plant Sci 10: 347-353 (2005)
Mol Cell 16: 498-500 (2004)
RNA 10: 1200-1214 (2004) - Selected by the Faculty of 1000
(www.facultyof1000.com/article/15247430/evaluation)
RNA 9: 1476-1490 (2003)
Genetics 163: 311-319 (2003)
| Chekanova Laboratory Department of Molecular Biology & Biochemistry |
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| Belostotsky-Chekanova Laboratory |