Chekanova Laboratory



Division of Molecular Biology & Biochemistry, School of Biological Sciences, University of Missouri - Kansas City.

University Lab Page

                 formerly Belostotsky-Chekanova Laboratory

 
 


Welcome to the Chekanova Laboratory

"Research efforts in the laboratory are directed towards developing a deeper understanding of various cellular RNA transactions, primarily using two model systems - bakers yeast Saccharomyces cerevisiae and higher plant Arabidopsis thaliana. We also continue to explore all scientific directions previously headed

by Dr. Dmitry Belostotsky (Current Opinion in Cell Biology Obituary, ASPB News Obituary)."


"Multiple quality control steps ensure that only fully processed and correctly assembled mRNP complexes are released from the site of transcription and exported into the cytoplasm. On the other hand, aberrant mRNPs are retained in the vicinity of the sites of transcription and ultimately degraded. Despite the widespread occurrence of such proofreading mechanisms from yeast to humans, mechanisms behind these quality control checkpoints remain poorly understood. On the yeast side of the lab, we seek to combine the yeast genetics and genomics with deconvolution microscopy-based imaging to obtain better insight into these processes."


"On the plant side, our efforts are directed towards understanding of the role of exosome complex in regulation of various steps of gene expression. The exosome complex is a conserved, functionally versatile macromolecular machine known to play a central and essential role in RNA metabolism both in the nucleus and in the cytoplasm. Using whole-transcriptome tiling microarrays we obtained first genome-wide exosome transcript maps which led to identification of a tightly repressed transcriptome that can only be visualized through transient inhibition of exosome activity. Our laboratory is pursuing further in depth examination of the role exosome complex plays in regulation of expression of this "hidden" layer of the transcriptome."

 

Publications

Shin JH, Chekanova JA. 2014 "Arabidopsis RRP6L1 and RRP6L2 function in FLOWERING LOCUS C silencing via regulation of antisense RNA synthesis",  PLOS Genetics, accepted.


Hsiao-Lin V. Wang, Brandon L. Dinwiddie, Herman Lee, Julia A. Chekanova. 2014. “Stress-induced siRNAs target the intro sequences that regulate splice site selection in Brachypodium” [Under review, 2014}.


El-Mellouki T., Pirani K., Belostotsky D.A., Chekanova J.A. . “Posttranscriptional perinuclear retention of activated genes in S. cerevisiae: chromatin-independent effects of Sus1 and position-dependent effects of H2A.Z” [RNA Revised, 2014]


Jun-Hye Shin*, Hsiao-Lin V. Wang*, Jinwon Lee, Brandon L. Dinwiddie, Dmitry A. Belostotsky, Julia A. Chekanova. 2013. “The Role of the Arabidopsis Exosome in siRNA–Independent Silencing of Heterochromatic Loci. PLOS Genetics”. Published online 28 March 2013. [PLOS Genetics]


Kelly SM, Leung SW, Apponi LH, Bramley AM, Tran EJ, Chekanova JA, Wente SR, Corbett AH.

Recognition of polyadenosine RNA by the zinc finger domain of nuclear poly(A) RNA-binding protein 2 (Nab2) is required for correct mRNA 3'-end formation. J Biol Chem. 2010 Aug 20;285(34):26022-32.


Gregory BD, Belostotsky DA. 2009. Whole-genome microarrays: applications and technical issues. Methods in Molecular Biology. [Pubmed]


Marom L, Hen-Avivi S, Pinchasi D, Chekanova JA, Belostotsky DA, Elroy-Stein O.. 2009. Diverse poly(A) binding proteins mediate internal translational initiation by a plant viral IRES. RNA Biology. Published online 7 September 2009. [Pubmed]


Belostotsky D.  2009. Exosome complex and pervasive transcription in eukaryotic genomes. Current Opinion in Cell Biology. Published online 19 May 2009. [Pubmed]


Belostotsky DA, Sieburth LE. 2008. Kill the messenger: mRNA decay and plant development. Current Opinion in Cell Biology. Published online 5 November 2008. [Pubmed]


Belostotsky DA. 2008. State of decay: an update on plant mRNA turnover. Current Topics in Microbiology and Immunology. [Pubmed]


Belostotsky D. 2008. Transcriptome targets of the exosome complex in plants.Methods in Enzymology. [Pubmed]


Chekanova JA, Abruzzi KC, Rosbash M, Belostotsky DA. 2008. Sus1, Sac3, and Thp1 mediate post-transcriptional tethering of active genes to the nuclear rim as well as to non-nascent mRNP. RNA. Published on 14 November 2007. [Pubmed]


Chekanova JA, Gregory BD, Reverdatto SV, Chen H, Kumar R, Hooker T, Yazaki J, Li P, Skiba N, Peng Q, Alonso J, Brukhin V, Grossniklaus U, Ecker JR, Belostotsky DA. 2007. Genome-wide high-resolution mapping of exosome substrates reveals hidden features in the Arabidopsis transcriptome. Cell. [Pubmed]


Abruzzi KC, Belostotsky DA, Chekanova JA, Dower K, Rosbash M. 2006. 3'-end formation signals modulate the association of genes with the nuclear periphery as well as mRNP dot formation. EMBO. [Pubmed]


Belostotsky DA, Rose AB. 2005. Plant gene expression in the age of systems biology: integrating transcriptional and post-transcriptional events. Trends in Plant Science. [Pubmed]


Belostotsky D. 2004. mRNA turnover meets RNA interference. Molecular Cell. [Pubmed]


Reverdatto SV, Dutko JA, Chekanova JA, Hamilton DA, Belostotsky DA. 2004. mRNA deadenylation by PARN is essential for embryogenesis in higher plants. RNA. Published online 9 July 2004. [Pubmed]


Chekanova JA, Belostotsky DA. 2003. Evidence that poly(A) binding protein has an evolutionarily conserved function in facilitating mRNA biogenesis and export. RNA. [Pubmed]


Belostotsky DA. 2003. Unexpected complexity of poly(A)-binding protein gene familiesin flowering plants: three conserved lineages that are at least 200 million years old and possible auto- and cross-regulation. Genetics. [Pubmed]

Last updated: November 11, 2014 10:50 AM