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SNP call rates and accuracies. a SNP call rates on single seed samples harvested 7, 15, 25, and 30 days after panicle initiation (DAPI), and leaf tissue. Each bar represents fourteen accessions replicated 5 times for each of ten independent KASP assays. b Genotypic concordance among single seed samples collected at 15, 25, and 30 DAPI relative to a reference sample generated from the leaf tissue. Each bar represents concordance rates for all 14 different accessions. c Call-rates for each SNP measured on 14 different accessions including leaf and single seeds samples collected at 15, 25, and 30 DAPI

Single seed-based sampling for MAS at scale. a Call rates from each SNP assay estimated using 1466 single seed and leaf samples. b Distributions of genotypic concordance for all 1466 accessions between single seed and leaf-based sampling strategies. c Call rate distribution across single seed and leaf samples for 1466 rice lines. Solid and dashed lines show the average call rates of 99.24% and 97.5% estimated on single seed and leaf-based samples respectively

Evaluation of single seed-based sampling at different developmental stages

Additionally, an in situ large-scale comparison between the seed-based sampling strategy and the current routine leaf-based sampling protocol was conducted by sampling single seeds and leaf punches from 1466 F6 plants from the head rows in the line stage testing (LST) breeding class of IRRI’s irrigated lowland rice breeding program that are routinely prioritized for MAS (Additional file 1: Table S1). Lines in the LST class were grown in 2-row plots with 6 plants per row spaced 40 cm from each other at IRRI’s ZES field facilities in Los Baños, Philippines. During the implementation of each sampling activity, the number of workers, time spent on each step, and operational costs were recorded to compare the relative difference in cost-effectiveness between the two protocols.

99%) and the high genotypic concordance results between single seed and leaf-based MAS strategies (

Based on observed CT values and genotypic results, the average amount of DNA extracted from a single seed (

In 2014, with co-investment and support from UWA and the Grains Research and Development Corporation (GRDC), the team began their research into a novel pulse platform.

Pure line (aSSD) plants look the same as each other, be similar in height and have the same yield.

Keeping a finger on the pulse

The predictability of offspring provides breeders with confidence to provide farmers with cultivars at a commercial scale. Farmers benefit with resilient crops that offer consistent yield at harvest time, fewer chemical treatments and reduced running costs. For consumers, a pure line seed results in food that is healthier and better tasting.

Commercial LED supplier Valoya have also integrated the research into their range of lighting systems also.

Working in close collaboration with breeders means that there is a faster release of novel varieties to the farmers.